The mechanism of chelator improved the tolerance and accumulation of poplar to Cd explored through differential expression protein based on iTRAQ.
Identifieur interne : 000067 ( Main/Exploration ); précédent : 000066; suivant : 000068The mechanism of chelator improved the tolerance and accumulation of poplar to Cd explored through differential expression protein based on iTRAQ.
Auteurs : Huiping Dai [République populaire de Chine] ; Shuhe Wei [République populaire de Chine] ; Azam Noori [États-Unis]Source :
- Journal of hazardous materials [ 1873-3336 ] ; 2020.
Abstract
Appropriate chelator may increase plant tolerance and accumulation for Cd in soil, but its molecular mechanism is unclear. In this experiment, the technology of isobaric tags for relative and absolute quantitation (iTRAQ) was used to compare the differential expression proteins (DEPs) and differential expression genes (DEGs) characteristics of poplar accumulating Cd combined with EDTA and/or EGTA. The results showed that the Cd concentrations, biomasses and activities of antioxidant enzymes of poplar were significantly increased in the treatments of chelator addition compared to the TCd. The number of co-intersecting specific proteins of TCd/CK, TCd+EDTA/TCd, TCd+EGTA/TCd and TCd+EDTA+EGTA/TCd was 49. Using the GO function and KEGG analysis, it was found that EDTA and EGTA might improve some main metabolic pathways of poplar leaves, which were involved in the enhancement of the expression of carbohydrate and energy metabolism-related proteins, regulation of cell energy metabolism, complementing and cooperating with each other in various ways, and dynamic regulation of energy metabolism. Particularly, chelator might induce the regulation of protein synthesis, folding and transport, and degradation of abnormal proteins in response to Cd toxicity. These results provided a theoretical basis for further elucidation of molecular mechanisms of poplar response to Cd stress.
DOI: 10.1016/j.jhazmat.2020.122370
PubMed: 32120214
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Electronic address: shuhewei@iae.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Pollution Ecology and Environment Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016</wicri:regionArea><wicri:noRegion>110016</wicri:noRegion></affiliation></author><author><name sortKey="Noori, Azam" sort="Noori, Azam" uniqKey="Noori A" first="Azam" last="Noori">Azam Noori</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Merrimack College, North Andover, MA, 01845, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Merrimack College, North Andover, MA, 01845</wicri:regionArea><wicri:noRegion>01845</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of hazardous materials</title><idno type="eISSN">1873-3336</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Appropriate chelator may increase plant tolerance and accumulation for Cd in soil, but its molecular mechanism is unclear. In this experiment, the technology of isobaric tags for relative and absolute quantitation (iTRAQ) was used to compare the differential expression proteins (DEPs) and differential expression genes (DEGs) characteristics of poplar accumulating Cd combined with EDTA and/or EGTA. The results showed that the Cd concentrations, biomasses and activities of antioxidant enzymes of poplar were significantly increased in the treatments of chelator addition compared to the T<sub>Cd.</sub> The number of co-intersecting specific proteins of T<sub>Cd</sub>/CK, T<sub>Cd+EDTA</sub>/T<sub>Cd</sub>, T<sub>Cd+EGTA</sub>/T<sub>Cd</sub> and T<sub>Cd+EDTA+EGTA</sub>/T<sub>Cd</sub> was 49. Using the GO function and KEGG analysis, it was found that EDTA and EGTA might improve some main metabolic pathways of poplar leaves, which were involved in the enhancement of the expression of carbohydrate and energy metabolism-related proteins, regulation of cell energy metabolism, complementing and cooperating with each other in various ways, and dynamic regulation of energy metabolism. Particularly, chelator might induce the regulation of protein synthesis, folding and transport, and degradation of abnormal proteins in response to Cd toxicity. These results provided a theoretical basis for further elucidation of molecular mechanisms of poplar response to Cd stress.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32120214</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3336</ISSN><JournalIssue CitedMedium="Internet"><Volume>393</Volume><PubDate><Year>2020</Year><Month>07</Month><Day>05</Day></PubDate></JournalIssue><Title>Journal of hazardous materials</Title><ISOAbbreviation>J Hazard Mater</ISOAbbreviation></Journal><ArticleTitle>The mechanism of chelator improved the tolerance and accumulation of poplar to Cd explored through differential expression protein based on iTRAQ.</ArticleTitle><Pagination><MedlinePgn>122370</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0304-3894(20)30358-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jhazmat.2020.122370</ELocationID><Abstract><AbstractText>Appropriate chelator may increase plant tolerance and accumulation for Cd in soil, but its molecular mechanism is unclear. In this experiment, the technology of isobaric tags for relative and absolute quantitation (iTRAQ) was used to compare the differential expression proteins (DEPs) and differential expression genes (DEGs) characteristics of poplar accumulating Cd combined with EDTA and/or EGTA. The results showed that the Cd concentrations, biomasses and activities of antioxidant enzymes of poplar were significantly increased in the treatments of chelator addition compared to the T<sub>Cd.</sub> The number of co-intersecting specific proteins of T<sub>Cd</sub>/CK, T<sub>Cd+EDTA</sub>/T<sub>Cd</sub>, T<sub>Cd+EGTA</sub>/T<sub>Cd</sub> and T<sub>Cd+EDTA+EGTA</sub>/T<sub>Cd</sub> was 49. Using the GO function and KEGG analysis, it was found that EDTA and EGTA might improve some main metabolic pathways of poplar leaves, which were involved in the enhancement of the expression of carbohydrate and energy metabolism-related proteins, regulation of cell energy metabolism, complementing and cooperating with each other in various ways, and dynamic regulation of energy metabolism. Particularly, chelator might induce the regulation of protein synthesis, folding and transport, and degradation of abnormal proteins in response to Cd toxicity. These results provided a theoretical basis for further elucidation of molecular mechanisms of poplar response to Cd stress.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Huiping</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Biological Science & Engineering, Shaanxi Province Key Laboratory of Bio-resources, Shaanxi University of Technology, Hanzhong, 723001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Shuhe</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Pollution Ecology and Environment Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China. Electronic address: shuhewei@iae.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noori</LastName><ForeName>Azam</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biology, Merrimack College, North Andover, MA, 01845, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Hazard Mater</MedlineTA><NlmUniqueID>9422688</NlmUniqueID><ISSNLinking>0304-3894</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Cd</Keyword><Keyword MajorTopicYN="Y">Chelator</Keyword><Keyword MajorTopicYN="Y">Differential expression protein (DEP)</Keyword><Keyword MajorTopicYN="Y">Isobaric tags for relative and absolute quantitation (iTRAQ)</Keyword><Keyword MajorTopicYN="Y">Poplar</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32120214</ArticleId><ArticleId IdType="pii">S0304-3894(20)30358-7</ArticleId><ArticleId IdType="doi">10.1016/j.jhazmat.2020.122370</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Dai, Huiping" sort="Dai, Huiping" uniqKey="Dai H" first="Huiping" last="Dai">Huiping Dai</name></noRegion><name sortKey="Wei, Shuhe" sort="Wei, Shuhe" uniqKey="Wei S" first="Shuhe" last="Wei">Shuhe Wei</name></country><country name="États-Unis"><noRegion><name sortKey="Noori, Azam" sort="Noori, Azam" uniqKey="Noori A" first="Azam" last="Noori">Azam Noori</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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